The reverse primer is designed so that the 5´finishes of The 2 primers anneal backto-again. B) Deletions are engineered by coming up with common, non-mutagenic ahead and reverse primers that flank the area to become deleted. C) Insertions fewer than or equal to six nucleotides are incorporated in to the https://site-directed-mutagenesis10853.vidublog.com/22355147/5-simple-statements-about-mutagenesis-explained